Unexpected Benefits of Replacing Phosphorothioates with Achiral Phosphordithioates for siRNA End Stabilization

Time: 1:30 pm
day: Track A Day 1 AM


  • Phosphordithioates (PS2s) were investigated as they could simplify oligonucleotide purification by replacing the undefined stereo centres introduced in phosphorothioate (PS) couplings
  • A single PS2 provides a similar strand stability as two PS
  • In vivo, PS2 linkages appears to introduce a longer duration of action than PS-containing siRNA with the same sequence